Depletion of the Mogat2 gene results in compensational overexpression of Dgat1 and Dgat2 genes. (a) Mogat1 gene mRNA was detected by qPCR in mammary tumors, stomach, small intestine, and colorectal tissues from Mogat2^+/+PyMT (n = 6) and Mogat2^−/−PyMT (n = 6) mice. As expected, Mogat1 gene expression was unaltered upon Mogat2 depletion. (b) In the same tissues, the Mogat2 expression level was suppressed in all the analyzed organs. (c) To compensate, Dgat1’s mRNA level was upregulated in the gastrointestinal system, especially in the small intestine, which is the major organ for fat absorption. (d) The level of Dgat2 mRNA had a similar overexpression pattern to that of Dgat1. (e, f) By analyzing TCGA data containing XXX cases of normal tissues adjacent to triple-negative breast adenocarcinoma, a significantly inverse correlation between MOGAT2 mRNA level and DGAT1/2 mRNA levels was found. Data represent the mean ± SEM, NS = non-significant, *P < 0.05, **P < 0.01 and ***P < 0.001.