Mammary tumor progression is accelerated by a high-fat diet regardless of Mogat2 gene depletion. (a) Kaplan–Meier curves showing the overall survival of Mogat2^+/+PyMT (n = 24) and Mogat2^−/−PyMT (n = 24) mice. Data were analyzed by the log-rank test. The mice were fed on a normal diet. (b) The appearance of mammary tumors in Mogat2^+/+PyMT (n = 19) and Mogat2^−/−PyMT (n = 20) mice fed with a high-fat diet (37% of calories from fat), and Mogat2^+/+PyMT (n = 15) fed on a normal diet were monitored by weekly palpation and are represented on Kaplan–Meier curves. (c) The tumor volume of all mammary glands was determined in Mogat2^+/+PyMT (n = 10) and Mogat2^−/−PyMT (n = 10) mice fed on a high-fat diet, as well as Mogat2^+/+PyMT (n = 10) mice fed on a normal diet which were measured weekly at 13–17 weeks old. Data represent the mean ± SEM. NS, non-significant; HFD, high-fat diet; ND, normal diet. *P < 0.05. **P < 0.01. ***P < 0.001.