Download original image
Migratory ability of NPC S18 cells is suppressed by knocking down SPINK6 expression. S18-NC and S18-SPINK6-KD cells (GFP-tagged) were seeded in a 96-well plate (2000 cells per well) and cultured in DMEM with 10% FBS in 5% CO2 at 37 °C for 24 h. Next, the plate was placed under a Nikon ECLIPSE Ti2 Inverted Microscope System supplied with 5% CO2 at 37 °C. Images of random fields at 100× magnification were taken automatically every 30 min for 72 h. A total of 145 sequential images of each field were taken, generating a video to display the movement of the cells at a speed of 15 FPS (frame/sec) (see Videos 1 and 2). The locations and moving tracks of the cells were analyzed using Image J software and the distance of movement between sequential images every 30 min were calculated. The videos displaying the movement of S18-NC and S18-SPINK6-KD cells are shown in Videos 1 and 2. The accumulated distance traveled in 72 h of 100 randomly selected cells from each cell line were calculated and are shown in A and B.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.