Open Access

Figure 1


Download original image

Schematic diagram of DSP workflow. 1. FFPE tissue sections mounted on slides are processed by standard IHC procedure and incubated with morphology marker reagents (fluorescence-labeled antibodies or dyes) and a mix of oligo conjugated antibodies. 2. High-quality images up to four channels of fluorescence are obtained. 3. ROIs are selected on the DSP instruments based on the morphological fluorescence marker. 4. Oligos in specific ROIs are released by UV light and collected by a microcapillary tube and dispensed into 96-well plates for subsequent processing. This oligo release and collection cycle is repeated for each ROI. 5. Digital counting by nCounter instruments to generate protein or RNA profiling data in a spatially resolved manner. Alternatively, highly multiplexed RNA profiling could be achieved using specific sequence adaptors (Unique Dual Index UDI) to allow NGS readout.

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.